[Experimental study of K562 cell apoptosis induced by siRNA.]

Posted by rob on February 27, 2005 under Uncategorized | Be the First to Comment

[Experimental study of K562 cell apoptosis induced by siRNA.]

Chen BB, Fan HH, Lin GW, Yuan ZH, Lu HZ, Gao L, Liu Y

Zhonghua Xue Ye Xue Za Zhi. 2004 Dec ; 25(12): 717-9

OBJECTIVES: To construct a siRNA expression vector pBCR6 that produces siRNA against bcr/abl mRNA and detect apoptosis rate of K562 cells after pBCR6 transfection. METHODS: Template sequence for siRNA was designed, synthesized and inserted into an expression vector pSilencer1.0-U6. Restriction analysis and sequencing were performed to verify the pBCR6 vector. Then pBCR6 was transfected into K562 cells by X-tremeGene Q2. pSilencer1.0-U6 was used as the control. At different time point after transfection, apoptosis rate was determined by Tunel and Annexin V+ PI with FCM. RESULT: pBCR6 was verified by restriction analysis and sequencing. The apoptosis rate of K562 cells markedly increased at 48 and 72 hour after transfected with pBCR6, and increased in a time-dependent manner [the apoptosis rate of transfected K562 cells was (47.80 +/- 1.63)% at 72 hrs, wheras the control group was (6.67 +/- 0.37)%, P

[Experimental study of K562 cell apoptosis induced by siRNA.]