A Worldwide Support Network For Chronic Myelogenous Leukemia
Posted by rob on April 24, 2005 under Uncategorized | 
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Mariza performs songs from her new album at Olga Cadaval Cultural Center in Sintra, a mountan village just outside of Lisbon. Portugal’s modern fado diva Mariza says she feels she has finally emerged from the shadows of Amalia, the queen of the nation’s melancholy music style who died in 1999 at the age of 79.
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Rodríguez-Flores J, Berzas Nevado JJ, Contento Salcedo AM, Cabello Díaz MP
J Chromatogr A. 2005 Mar 11; 1068(1): 175-82
The viability of nonaqueous capillary electrophoresis (NACE) was investigated for determination of gleevec and its main metabolite in human urine using a fused-silica capillary. Baseline separation of the studied solutes was obtained using a nonaqueous solution composed of 12 mM ammonium acetate and 87.6 mM acetic acid in methanol-acetonitrile (ACN) (80:20, v:v) providing analysis time shorter than 3 min. Different aspects including stability of the solutions, linearity, accuracy and precision were studied in order to validate the method in the urine matrix. Detection limits of 24 microg L(-1) for gleevec and its metabolite were obtained. A robustness test of the method was carried out using the Plackett-Burman fractional factorial model with a matrix of 15 experiments. The developed method is simple, rapid and sensitive and has been used to determine gleveec and its metabolite at clinically relevant levels in human urine. Before NACE determination, a solid-phase extraction (SPE) procedure with a C18 cartridge was necessary. Real determination of these analytes in two patient urines were done.
