Role of exosomes released by chronic myelogenous leukemia cells in angiogenesis

Posted by rob on June 15, 2011 under Uncategorized | Comments are off for this article

AbstractThe present study is designed to assess if exosomes released from Chronic Myelogenous Leukemia (CML) cells may modulate angiogenesis. We have isolated and characterized the exosomes generated from LAMA84 CML cells and demonstrated that addition of exosomes to human vascular endothelial cells (HUVEC) induces an increase of both ICAM?1 and VCAM?1 cell adhesion molecules and interleukin?8 expression. The stimulation of cell?cell adhesion molecules was paralleled by a dose?dependent increase of adhesion of CML cells to a HUVEC monolayer. We further showed that the treatment with exosomes from CML cells caused an increase in endothelial cell motility accompanied by a loss of VE?cadherin and ??catenin from the endothelial cell surface. Functional characterization of exosom…
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